Training

Experiome  /  Training

Training Modules

TRAINING

At Experiome, we give outstanding importance on excellent training in our thoroughly operational biotechnology laboratory.

The training modules are designed keeping in view the continuously evolving biotechnology education and research scenario of the country.

The ultimate goal of our training programs is to generate appropriately trained human resource that can contribute to the growth and development of biotechnology, specifically, and life sciences, in general.

TRAINING MODULES

Wet lab modules

No. Training module Duration Price
1.1 Introduction to a biotechnology laboratory
1.2 Good Lab Practices for a biotechnology laboratory
1.3 Introduction to biotechnology laboratory instruments (Weighing balance, Autoclave, water bath, Dry bath, Gel electrophoresis system, Laminar flow cabinet, Gel documentation system, UV- visible double beam spectrophotometer, Vortex mixer, incubator, PCR Cycler, Centrifuge, -20° freezer, pH meter)
1.4 Instrumentation and workflow in a biotechnology laboratory: Usage and handling of different instruments
1.5 Practical applications of mole concept; and pipette handling in a biotechnology laboratory
1.6 pH meter: Fundamentals and applications of hydrogen ion concentration (pH) in making buffer solutions
1.7 Autoclaving and working in a laminar flow: Fundamentals and applications
2.1 Introduction to recombinant DNA technology: Fundamentals and applications
2.2 Methods of Bacterial Culture
2.3 Plasmid DNA Isolation (Alkaline Lysis Method)
2.4 Plant DNA Extraction (CTAB method)
2.5 Plant RNA Extraction (TRIZOL Method)
2.6
Competent Cell preparation (CaCl2 and MgCl2 Method) and transformation of E. coli cells
2.7 Screening of transformed cells: Blue-white selection
2.8 PCR for the amplification of DNA fragments
2.9 Agarose Gel electrophoresis for DNA separation
2.10 SDS-PAGE for protein separation
3 Cloning of a gene from E. coli(prokaryotic system) 3 months 17,000
3.1 Fundamentals of gene cloning
3.2 Selection of gene of interest from E. coli genome (Literature survey)
3.3 Designing of primers for gene of interest
3.4 Genomic DNA isolation from E. coli using CTAB method
3.5 Amplification of desired gene through PCR
3.6 Agarose gel electrophoresis to separate the desired DNA band and its elution
3.7 Integration of PCR-amplified gene into a cloning vector (Restriction digestion and ligation reaction)
3.8 Transformation of cloned gene in E. coli competent cells
3.9 Screening of transformed E. coli cells
3.10 Plasmid DNA isolation from E. coli cells
3.11 Sequence confirmation of cloned gene through Sanger sequencing
3.12 Documentation and presentation of the results obtained during the module
4 Cloning of a gene/insert into a vector and confirmation by restriction digestion and Sanger sequencing (Eukaryotic system) 6 months 38,000
4.1 Fundamentals of gene cloning
4.2 Selection of gene of interest from eukaryotic system (Plant) (Literature survey)
4.3 Designing of primers for gene of interest
4.4 RNA extraction through TRIZOL method
4.5 Conversion of RNA into cDNA through reverse transcription using Reverse Transcriptase enzyme
4.6 Amplification of gene of interest through PCR
4.7 Agarose gel electrophoresis to separate the desired DNA band
4.8 Cutting of desired band and its elution
4.9 Transfer of eluted gene of interest into a cloning vector (Restriction digestion and ligation reaction)
4.10 Competent cell preparation
4.11 Transformation of cloned gene in E. coli competent cells
4.12 Culture of transformed E. coli in LB media
4.13 Plasmid DNA isolation from transformed E. coli
4.14 Confirmation of result through Sanger sequencing
4.15 Documentation and presentation of the results obtained during the module
5 Characterization of a gene family by semiquantitative RT-PCR 6 months 38,000
5.1 Introduction to gene families
5.2 Fundamentals of bioinformatics tools used for gene family characterization
5.3 Design primer for the gene of interest for selected gene family to be analyzed
5.4 RNA Extraction through TRIZOL method
5.5 Convert RNA to cDNA through reverse transcriptase
5.6 Perform semi-quantitative RT-PCR to amplify desired genes
5.7 Agarose gel electrophoresis to separate the amplified genes
5.8 Data visualization through GelDoc system
5.9 Documentation and presentation of the results obtained during the module
6 Cloning of a gene promoter 3 months 17,000
6.1 Selection of promoter region to be cloned
6.2 Retrieval and analysis of promoter sequences using in silico approach
6.3 Designing of primers for the selected promoter sequences
6.4 Amplify promoter sequences through PCR
6.5 Run PCR sample on agarose gel
6.6 Visualization of band through GelDoc system
6.7 Documentation and presentation of the results obtained during the module
7 DNA fingerprinting 1 month 9000
7.1 Introduction and applications of DNA Fingerprinting
7.2 Different methods of DNA Fingerprinting
7.3 Isolation of genomic DNA
7.4 Perform PCR with RAPD primers
7.5 Run the PCR samples on agarose gel
7.6 Visualization of DNA bands through GelDoc system
7.7 Documentation and presentation of the results obtained during the module
8 Protein isolation and analysis 15 days 6000
8.1 To perform seed sterilization and germination
8.2 Protein extraction from seedlings
8.3 Bradford Protein Assay
8.4 Protein separation by SDS- PAGE
8.5 Documentation and presentation of the skills learnt during the module
9 Protein Expression 3 months 17,000
9.1 Introduction to gene expression vectors and systems
9.2 Prepare competent cells for transformation
9.3 Transformation of expression vector containing the desired gene into competent E. coli cells
9.4 Bacterial culture
9.5 Extraction of protein from cells in suspensions through cell lysis
9.6 Protein separation through SDS-PAGE
9.7 Documentation and presentation of the skills learnt during the module
10 Microbiology Module 1 month 7000
10.1 Preparation of different microbial culture media
10.2 Microbial isolation from natural sources (air, water, soil)
10.3 Serial dilution
10.4 Streaking and spreading of microbes
10.5 Inoculation of microbes from plate to liquid media (LB)
10.6 Bacterial growth kinetic analysis
10.7 Biophysical testing of microbes (Temperature, Salt)
10.8 Biochemical testing of microbes
10.9 Gram staining
10.10 Antibiotic sensitivity test
10.11 Maintenance & storage of E.coli cells
10.12 Documentation and presentation of the skills learnt during the module
11 Customized training module Depend on the scope and dimension of the module Depend on the scope and dimension of the module

Bioinformatics modules

No. Training module Duration Price
1 Basic bioinformatics toolkit for biological sequence manipulation 15 days 3500
1.1 Basic Bioinformatics toolkit for biological sequence manipulation (15 days)
1.2 Introduction to bioinformatics and its applications
1.3 Different file formats used in bioinformatics
1.4 Prerequisites for BLAST search and its applications
1.5 Biological sequence retrieval from different databases
1.5 Sequence alignment through Clustal Omega
1.6 Conserved residue depiction through ESPript
1.7 Conserved motif depiction through MEME
1.8 Phylogenetic tree construction through MEGA7
1.9 Documentation and presentation of the skills learnt during the module
2 An overview of bioinformatics database 15 days 3500
2.1 Introduction to database: Characteristics and applications of database
2.2 Bioinformatics database:Different kinds of sequence and structure database and their significance
2.3 Introduction to NCBI, EMBL, DDBJ, Ensembl databases etc.
2.4 Whole genome database
2.5 KEGG Pathwaydatabase for metabolic pathways
2.6 Protein structure database: Protein Data Bank
2.7 Chemical structure database: PubChem
2.8 Documentation and presentation of the skills learnt during the module
3 In silico characterization of a gene family 6 months 21000
3.1 Introduction to the concept of gene families
3.2 BLAST search for desired gene familyfor selected species on its respective database
3.3 Retrieval of sequences after BLAST search in FASTA format
3.4 Conserved domain analysis for validation of results through NCBI-CD, Pfam and SMART databases
3.5 Diagrammatic representation of conserved domains through offline Illustrator tool
3.6 Protein physicochemical characterizationusing ProtParam tool by Expasy
3.7 Sub cellular localization predictionusing various independent tools (CELLO, WolfPSort, TargetP, DeepLoc, ChloroP)
3.8 Chromosomal localization depiction through TBtools
3.9 Gene duplication analysis through PAL2NAL server
3.10 Phylogenetic analysis using MEGA.X and MEGA.7 tools
3.11 Conserved motif analysis using MEME suite
3.12 Gene architecture analysis through Gene Structure Display Server (GSDS Tool)
3.13 Multiple sequence alignment using Clustal omega
3.14 Conserved residue depiction using ESPript tool
3.15 Cis- regulatory element analysis using PLANTCARE Tool
3.16 Phosphorylation and glycosylation site prediction
3.17 Interactome analysis using STRING tool
3.18 Documentation and presentation of the results obtained during the module
4 Next generation sequencing (NGS) data analysis 3 months 14000
4.1 Introduction to NGS and its applications
4.2 Fundamentals of different NGS platforms and methods
4.3 Introduction to diverse NGS platforms
4.4 Different NGS data file formats
4.5 Genome assembly algorithms
4.6 Overview of NGS workflow
4.7 Use of Gene Expression Omnibus (GEO) for functional genomics
4.8 NGS data analysis and presentation
4.9 Documentation and presentation of the results obtained during the module
5 Proteomics data analysis 1 month 6500
5.1 Protein structure prediction
5.2 Protein-protein interaction analysis
5.3 3D- Structure prediction and modeling
5.4 Validation of predicted 3D structure model
5.5 Post translational modifications
5.6 Peptide mass finger print: Fundamentals and applications
5.7 Concept of MOWSE (MOlecular Weight SEarch) Score
5.8 Mascot Server for MS/MS data
6 Computer-aided drug designing 3 months 15000
6.1 Introduction to drug discovery and design
6.2 Fundamentals of ligands and receptors
6.3 Concepts of molecular docking
6.4 Structure based drug design (target-ligand docking)
6.5 Pharmacophore Modelling
6.6 QSAR analysis
6.7 Insilico Screening (ADME)
6.8 Personalized medicine and its implications
6.9 Documentation and presentation of the results obtained during the module
7 Prediction of cis-elements and transcription factor binding sites 3 months 14000
7.1 Introduction to transcription factor biology
7.2 Retrieval of promoter region sequences
7.3 Cis-regulatory element analysis from multiple species
7.4 Comparative analysis of promoters from multiple species
7.5 Transcription factor binding site prediction through online available tools
7.6 Documentation and presentation of the results obtained during the module
8 CRISPR-Cas vector designing for precise genome editing 1 month 6500
8.1 Introduction to precise genome editing
8.2 Fundamentals and applications of CRISPR technology
8.3 Introduction to CRISPR bioinformatics
8.4 CRISPR Design Experiment-Strategizing the editing
8.5 Designing of guide RNAs using bioinformatics tools
8.6 Genome-wide CRISPR screens
8.7 CRISPR and Genetic Engineering
8.8 Future of CRISPR-Challenges and Research outcome
9 Microarray data analysis 3 month 14000
9.1 Identification of differential gene expression from two different datasets such as normal or diseased
9.2 Cluster analysis
9.3 Classification analysis
9.4 Protein-protein interaction network and pathway analysis
9.5 Gene set enrichment analysis
9.6 Documentation and presentation of the results obtained during the module
10 Ingenuity Pathway Analysis (IPA) for metabolic pathway analysis 1 month 6500
10.1 Interpretation and visualization of data derived from omics experiments like microarray, NGS, RNA-seq etc.
11 Customized training module Depend on the scope and dimension of the module Depend on the scope and dimension of the module

Skill enhancement modules

No. Training module Duration Price
1 Scientific writing and literature survey 3 months 17000
2 MS Excel and MS PowerPoint for statistical data analysis and presentation 15 days 3500
3 BioRender for making publication-ready professional life sciences figures 15 days 3500
4 GraphPad PRISM for making publication-ready professional graphs 1 months 6500
5 Customized training module Depend on the scope and dimension of the moduleh Depend on the scope and dimension of the module